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    Qiagen endofree plasmid purification columns
    Endofree Plasmid Purification Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endofree+columns/endofree+plasmid+maxi+kit/pm39418312-86-6-10
    Average 90 stars, based on 1 article reviews
    endofree plasmid purification columns - by Bioz Stars, 2026-10
    90/100 stars

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    Plasmid Preparation:

    Article Title: Cooperation of Adenosine with Macrophage Toll-4 Receptor Agonists Leads to Increased Glycolytic Flux through the Enhanced Expression of PFKFB3 Gene
    Article Snippet: .. All plasmid DNA was purified using EndoFree columns (Qiagen). .. Subconfluent Raw 264.7 cells were seeded in 12-well plates and transfected on the following day with 600 ng of plasmid DNA by using the FuGENE 6 reagent (Roche Applied Science), according to the manufacturer's recommendations.

    Article Title: NOXA-Induced Alterations in the Bax/Smac Axis Enhance Sensitivity of Ovarian Cancer Cells to Cisplatin
    Article Snippet: The primary antibodies for western blotting are as follows: Anti-Bcl-2 (sc-130307), anti-Bcl-x L (sc-8392), anti-Mcl-1 (sc-69839), anti-NOXA (sc-30209), anti-p53 (DO-1), Anti-p73 (H-79), anti-p21 Waf1/Cip1 and anti–β-actin (Santa Cruz, CA); anti-Bax N-20 (sc-493); anti–caspase-3, anti–cleaved caspase-3, anti–caspase-9 and its cleaved form (Cell Signaling Technology, Danvers, MA); anti-Smac (clone FKE02, R&D Systems, Minneapolis, MN); anti-Cyt C (sc-13156), cytochrome oxidase subunit IV (Molecular Probes). .. Both pcDNA3.1(pc3.1) and pcDNA3.1 plasmid encoding Human NOXA gene (pcDNA3.1-hNOXA, pc3.1-hNoxa) were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously . pc3.1-Bax constructs were generated according to our previous methods . .. Small interfering RNA (siRNA) oligonucleotides were purchased from Dharmacon (Lafayette, CO) with sequences targeting Bax (5′-AACUGAUCAGA ACCAUCAUGG-3′) and Smac (5′-AACCCUGUGUGCGGUUCCUAU-3′).

    Purification:

    Article Title: Cooperation of Adenosine with Macrophage Toll-4 Receptor Agonists Leads to Increased Glycolytic Flux through the Enhanced Expression of PFKFB3 Gene
    Article Snippet: .. All plasmid DNA was purified using EndoFree columns (Qiagen). .. Subconfluent Raw 264.7 cells were seeded in 12-well plates and transfected on the following day with 600 ng of plasmid DNA by using the FuGENE 6 reagent (Roche Applied Science), according to the manufacturer's recommendations.

    Article Title: PNAS-4an Early DNA Damage Response Gene, Induces S Phase Arrest and Apoptosis by Activating Checkpoint Kinases in Lung Cancer Cells
    Article Snippet: .. Eukaryotic expression vectors for expressing wild-type hChk1 and truncated hChk1 mutant (residues 1–299) tagged with Myc at the N terminus were generated into pTango-zeo-N3Myc (pTNM) vector and defined as pTango-zeo-N3Myc-Chk1[M-hChk1(WT)] and pTango-zeo-N3My c-Chk1-T[M-hChk1-T]. pcDNA3.1 (pc3.1), pcDNA3.1-GFP (pc3.1-GFP), pTNM, M-hChk1(WT), M-hChk1-T, and pc3.1- hPNAS-4 plasmids were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously ( 12 ). .. Reagents The following antibodies were used: the goat anti-PPPDE1/PNAS-4 Antibody (Everest Biotech, Ltd.), anti-p53, anti-p53 (Ser-15), anti-p21 Waf1/Cip1 , anti- p27 Kip1 , anti-p16 INK4a , anti-Cdc25A, anti-CDK2, anti-phospho-CDK2 (Tyr-15), anti-cyclin A, anti-cyclin E, anti-cyclin D1, anti-cyclin B1, anti-CDK4, anti-CDK6, anti-Myc, anti-Chk1, anti-Chk2, anti-phospho-Chk1 (Ser-345), anti-phospho-Chk2 (Thr-68), anti-ATM, anti-phospho-ATM (Ser-1981), and anti-β-actin (Santa Cruz Biotechnology, Inc.); anti-DNA-PKcs, anti-phospho-DNA-PKcs (Thr-2609), and anti-ATX (Abcam, Cambridge, MA); anti-ERK, anti-phospho-ERK, anti-caspase-3, and anti-caspase-7 (Cell Signaling Technology, Danvers, MA); and anti-γ-H2AX (Ser-139) (Abcam).

    Article Title: RNA interference-mediated silencing of focal adhesion kinase inhibits growth of human colon carcinoma xenograft in nude mice.
    Article Snippet: Focal adhesion kinase (FAK), which plays a pivotal role in mediating cell proliferation, survival and migration, is frequently overexpressed in human colon cancer.. In the present study, we utilized the short hairpin RNA (shRNA) to knock down the expression of FAK in SW480 human colon cancer cells in vitro.. Furthermore, nude mice bearing human colon carcinoma SW480 were established and treated with plasmids encoding FAK shRNA encapsulated in DOTAP: Chol cationic liposome through tail vein injection.

    Article Title: PNAS-4, an Early DNA Damage Response Gene, Induces S Phase Arrest and Apoptosis by Activating Checkpoint Kinases in Lung Cancer Cells
    Article Snippet: .. Eukaryotic expression vectors for expressing wild-type hChk1 and truncated hChk1 mutant (residues 1–299) tagged with Myc at the N terminus were generated into pTango-zeo- N3Myc (pTNM) vector and defined as pTango-zeo-N3MycChk1[M-hChk1(WT)] and pTango-zeo-N3My c-Chk1-T[MhChk1-T]. pcDNA3.1 (pc3.1), pcDNA3.1-GFP (pc3.1-GFP), pTNM, M-hChk1(WT), M-hChk1-T, and pc3.1- hPNAS-4 plasmids were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously (12). .. Reagents—The following antibodies were used: the goat antiPPPDE1/PNAS-4 Antibody (Everest Biotech, Ltd.), anti-p53, anti-p53 (Ser-15), anti-p21Waf1/Cip1, anti- p27Kip1, antip16INK4a, anti-Cdc25A, anti-CDK2, anti-phospho-CDK2 (Tyr15), anti-cyclin A, anti-cyclin E, anti-cyclin D1, anti-cyclin B1, anti-CDK4, anti-CDK6, anti-Myc, anti-Chk1, anti-Chk2, antiphospho-Chk1 (Ser-345), anti-phospho-Chk2 (Thr-68), antiATM, anti-phospho-ATM (Ser-1981), and anti- -actin (Santa Cruz Biotechnology, Inc.); anti-DNA-PKcs, anti-phosphoDNA-PKcs (Thr-2609), and anti-ATX (Abcam, Cambridge, MA); anti-ERK, anti-phospho-ERK, anti-caspase-3, and anticaspase-7 (Cell Signaling Technology, Danvers, MA); and anti-H2AX (Ser-139) (Abcam).

    Article Title: Interleukin-1 and tumor necrosis factor-αmodulators; syntheses of such modulators and methods of using such modulators
    Article Snippet: .. Plasmids were purified using EndoFree Qiagen columns. ..

    Article Title: NOXA-Induced Alterations in the Bax/Smac Axis Enhance Sensitivity of Ovarian Cancer Cells to Cisplatin
    Article Snippet: The primary antibodies for western blotting are as follows: Anti-Bcl-2 (sc-130307), anti-Bcl-x L (sc-8392), anti-Mcl-1 (sc-69839), anti-NOXA (sc-30209), anti-p53 (DO-1), Anti-p73 (H-79), anti-p21 Waf1/Cip1 and anti–β-actin (Santa Cruz, CA); anti-Bax N-20 (sc-493); anti–caspase-3, anti–cleaved caspase-3, anti–caspase-9 and its cleaved form (Cell Signaling Technology, Danvers, MA); anti-Smac (clone FKE02, R&D Systems, Minneapolis, MN); anti-Cyt C (sc-13156), cytochrome oxidase subunit IV (Molecular Probes). .. Both pcDNA3.1(pc3.1) and pcDNA3.1 plasmid encoding Human NOXA gene (pcDNA3.1-hNOXA, pc3.1-hNoxa) were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously . pc3.1-Bax constructs were generated according to our previous methods . .. Small interfering RNA (siRNA) oligonucleotides were purchased from Dharmacon (Lafayette, CO) with sequences targeting Bax (5′-AACUGAUCAGA ACCAUCAUGG-3′) and Smac (5′-AACCCUGUGUGCGGUUCCUAU-3′).

    Article Title: CXCL10 enhances radiotherapy effects in HeLa cells through cell cycle redistribution
    Article Snippet: .. The plasmids were purified through two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA, USA) according to the manufacturer’s instructions. ..

    Article Title: Interleukin-1 and tumor necrosis factor-α modulators; syntheses of such modulators and methods of using such modulators
    Article Snippet: .. Plasmids were purified using EndoFree Qiagen columns. ..

    Expressing:

    Article Title: PNAS-4an Early DNA Damage Response Gene, Induces S Phase Arrest and Apoptosis by Activating Checkpoint Kinases in Lung Cancer Cells
    Article Snippet: .. Eukaryotic expression vectors for expressing wild-type hChk1 and truncated hChk1 mutant (residues 1–299) tagged with Myc at the N terminus were generated into pTango-zeo-N3Myc (pTNM) vector and defined as pTango-zeo-N3Myc-Chk1[M-hChk1(WT)] and pTango-zeo-N3My c-Chk1-T[M-hChk1-T]. pcDNA3.1 (pc3.1), pcDNA3.1-GFP (pc3.1-GFP), pTNM, M-hChk1(WT), M-hChk1-T, and pc3.1- hPNAS-4 plasmids were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously ( 12 ). .. Reagents The following antibodies were used: the goat anti-PPPDE1/PNAS-4 Antibody (Everest Biotech, Ltd.), anti-p53, anti-p53 (Ser-15), anti-p21 Waf1/Cip1 , anti- p27 Kip1 , anti-p16 INK4a , anti-Cdc25A, anti-CDK2, anti-phospho-CDK2 (Tyr-15), anti-cyclin A, anti-cyclin E, anti-cyclin D1, anti-cyclin B1, anti-CDK4, anti-CDK6, anti-Myc, anti-Chk1, anti-Chk2, anti-phospho-Chk1 (Ser-345), anti-phospho-Chk2 (Thr-68), anti-ATM, anti-phospho-ATM (Ser-1981), and anti-β-actin (Santa Cruz Biotechnology, Inc.); anti-DNA-PKcs, anti-phospho-DNA-PKcs (Thr-2609), and anti-ATX (Abcam, Cambridge, MA); anti-ERK, anti-phospho-ERK, anti-caspase-3, and anti-caspase-7 (Cell Signaling Technology, Danvers, MA); and anti-γ-H2AX (Ser-139) (Abcam).

    Article Title: PNAS-4, an Early DNA Damage Response Gene, Induces S Phase Arrest and Apoptosis by Activating Checkpoint Kinases in Lung Cancer Cells
    Article Snippet: .. Eukaryotic expression vectors for expressing wild-type hChk1 and truncated hChk1 mutant (residues 1–299) tagged with Myc at the N terminus were generated into pTango-zeo- N3Myc (pTNM) vector and defined as pTango-zeo-N3MycChk1[M-hChk1(WT)] and pTango-zeo-N3My c-Chk1-T[MhChk1-T]. pcDNA3.1 (pc3.1), pcDNA3.1-GFP (pc3.1-GFP), pTNM, M-hChk1(WT), M-hChk1-T, and pc3.1- hPNAS-4 plasmids were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously (12). .. Reagents—The following antibodies were used: the goat antiPPPDE1/PNAS-4 Antibody (Everest Biotech, Ltd.), anti-p53, anti-p53 (Ser-15), anti-p21Waf1/Cip1, anti- p27Kip1, antip16INK4a, anti-Cdc25A, anti-CDK2, anti-phospho-CDK2 (Tyr15), anti-cyclin A, anti-cyclin E, anti-cyclin D1, anti-cyclin B1, anti-CDK4, anti-CDK6, anti-Myc, anti-Chk1, anti-Chk2, antiphospho-Chk1 (Ser-345), anti-phospho-Chk2 (Thr-68), antiATM, anti-phospho-ATM (Ser-1981), and anti- -actin (Santa Cruz Biotechnology, Inc.); anti-DNA-PKcs, anti-phosphoDNA-PKcs (Thr-2609), and anti-ATX (Abcam, Cambridge, MA); anti-ERK, anti-phospho-ERK, anti-caspase-3, and anticaspase-7 (Cell Signaling Technology, Danvers, MA); and anti-H2AX (Ser-139) (Abcam).

    Mutagenesis:

    Article Title: PNAS-4an Early DNA Damage Response Gene, Induces S Phase Arrest and Apoptosis by Activating Checkpoint Kinases in Lung Cancer Cells
    Article Snippet: .. Eukaryotic expression vectors for expressing wild-type hChk1 and truncated hChk1 mutant (residues 1–299) tagged with Myc at the N terminus were generated into pTango-zeo-N3Myc (pTNM) vector and defined as pTango-zeo-N3Myc-Chk1[M-hChk1(WT)] and pTango-zeo-N3My c-Chk1-T[M-hChk1-T]. pcDNA3.1 (pc3.1), pcDNA3.1-GFP (pc3.1-GFP), pTNM, M-hChk1(WT), M-hChk1-T, and pc3.1- hPNAS-4 plasmids were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously ( 12 ). .. Reagents The following antibodies were used: the goat anti-PPPDE1/PNAS-4 Antibody (Everest Biotech, Ltd.), anti-p53, anti-p53 (Ser-15), anti-p21 Waf1/Cip1 , anti- p27 Kip1 , anti-p16 INK4a , anti-Cdc25A, anti-CDK2, anti-phospho-CDK2 (Tyr-15), anti-cyclin A, anti-cyclin E, anti-cyclin D1, anti-cyclin B1, anti-CDK4, anti-CDK6, anti-Myc, anti-Chk1, anti-Chk2, anti-phospho-Chk1 (Ser-345), anti-phospho-Chk2 (Thr-68), anti-ATM, anti-phospho-ATM (Ser-1981), and anti-β-actin (Santa Cruz Biotechnology, Inc.); anti-DNA-PKcs, anti-phospho-DNA-PKcs (Thr-2609), and anti-ATX (Abcam, Cambridge, MA); anti-ERK, anti-phospho-ERK, anti-caspase-3, and anti-caspase-7 (Cell Signaling Technology, Danvers, MA); and anti-γ-H2AX (Ser-139) (Abcam).

    Article Title: PNAS-4, an Early DNA Damage Response Gene, Induces S Phase Arrest and Apoptosis by Activating Checkpoint Kinases in Lung Cancer Cells
    Article Snippet: .. Eukaryotic expression vectors for expressing wild-type hChk1 and truncated hChk1 mutant (residues 1–299) tagged with Myc at the N terminus were generated into pTango-zeo- N3Myc (pTNM) vector and defined as pTango-zeo-N3MycChk1[M-hChk1(WT)] and pTango-zeo-N3My c-Chk1-T[MhChk1-T]. pcDNA3.1 (pc3.1), pcDNA3.1-GFP (pc3.1-GFP), pTNM, M-hChk1(WT), M-hChk1-T, and pc3.1- hPNAS-4 plasmids were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously (12). .. Reagents—The following antibodies were used: the goat antiPPPDE1/PNAS-4 Antibody (Everest Biotech, Ltd.), anti-p53, anti-p53 (Ser-15), anti-p21Waf1/Cip1, anti- p27Kip1, antip16INK4a, anti-Cdc25A, anti-CDK2, anti-phospho-CDK2 (Tyr15), anti-cyclin A, anti-cyclin E, anti-cyclin D1, anti-cyclin B1, anti-CDK4, anti-CDK6, anti-Myc, anti-Chk1, anti-Chk2, antiphospho-Chk1 (Ser-345), anti-phospho-Chk2 (Thr-68), antiATM, anti-phospho-ATM (Ser-1981), and anti- -actin (Santa Cruz Biotechnology, Inc.); anti-DNA-PKcs, anti-phosphoDNA-PKcs (Thr-2609), and anti-ATX (Abcam, Cambridge, MA); anti-ERK, anti-phospho-ERK, anti-caspase-3, and anticaspase-7 (Cell Signaling Technology, Danvers, MA); and anti-H2AX (Ser-139) (Abcam).

    Generated:

    Article Title: PNAS-4an Early DNA Damage Response Gene, Induces S Phase Arrest and Apoptosis by Activating Checkpoint Kinases in Lung Cancer Cells
    Article Snippet: .. Eukaryotic expression vectors for expressing wild-type hChk1 and truncated hChk1 mutant (residues 1–299) tagged with Myc at the N terminus were generated into pTango-zeo-N3Myc (pTNM) vector and defined as pTango-zeo-N3Myc-Chk1[M-hChk1(WT)] and pTango-zeo-N3My c-Chk1-T[M-hChk1-T]. pcDNA3.1 (pc3.1), pcDNA3.1-GFP (pc3.1-GFP), pTNM, M-hChk1(WT), M-hChk1-T, and pc3.1- hPNAS-4 plasmids were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously ( 12 ). .. Reagents The following antibodies were used: the goat anti-PPPDE1/PNAS-4 Antibody (Everest Biotech, Ltd.), anti-p53, anti-p53 (Ser-15), anti-p21 Waf1/Cip1 , anti- p27 Kip1 , anti-p16 INK4a , anti-Cdc25A, anti-CDK2, anti-phospho-CDK2 (Tyr-15), anti-cyclin A, anti-cyclin E, anti-cyclin D1, anti-cyclin B1, anti-CDK4, anti-CDK6, anti-Myc, anti-Chk1, anti-Chk2, anti-phospho-Chk1 (Ser-345), anti-phospho-Chk2 (Thr-68), anti-ATM, anti-phospho-ATM (Ser-1981), and anti-β-actin (Santa Cruz Biotechnology, Inc.); anti-DNA-PKcs, anti-phospho-DNA-PKcs (Thr-2609), and anti-ATX (Abcam, Cambridge, MA); anti-ERK, anti-phospho-ERK, anti-caspase-3, and anti-caspase-7 (Cell Signaling Technology, Danvers, MA); and anti-γ-H2AX (Ser-139) (Abcam).

    Article Title: PNAS-4, an Early DNA Damage Response Gene, Induces S Phase Arrest and Apoptosis by Activating Checkpoint Kinases in Lung Cancer Cells
    Article Snippet: .. Eukaryotic expression vectors for expressing wild-type hChk1 and truncated hChk1 mutant (residues 1–299) tagged with Myc at the N terminus were generated into pTango-zeo- N3Myc (pTNM) vector and defined as pTango-zeo-N3MycChk1[M-hChk1(WT)] and pTango-zeo-N3My c-Chk1-T[MhChk1-T]. pcDNA3.1 (pc3.1), pcDNA3.1-GFP (pc3.1-GFP), pTNM, M-hChk1(WT), M-hChk1-T, and pc3.1- hPNAS-4 plasmids were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously (12). .. Reagents—The following antibodies were used: the goat antiPPPDE1/PNAS-4 Antibody (Everest Biotech, Ltd.), anti-p53, anti-p53 (Ser-15), anti-p21Waf1/Cip1, anti- p27Kip1, antip16INK4a, anti-Cdc25A, anti-CDK2, anti-phospho-CDK2 (Tyr15), anti-cyclin A, anti-cyclin E, anti-cyclin D1, anti-cyclin B1, anti-CDK4, anti-CDK6, anti-Myc, anti-Chk1, anti-Chk2, antiphospho-Chk1 (Ser-345), anti-phospho-Chk2 (Thr-68), antiATM, anti-phospho-ATM (Ser-1981), and anti- -actin (Santa Cruz Biotechnology, Inc.); anti-DNA-PKcs, anti-phosphoDNA-PKcs (Thr-2609), and anti-ATX (Abcam, Cambridge, MA); anti-ERK, anti-phospho-ERK, anti-caspase-3, and anticaspase-7 (Cell Signaling Technology, Danvers, MA); and anti-H2AX (Ser-139) (Abcam).

    Article Title: NOXA-Induced Alterations in the Bax/Smac Axis Enhance Sensitivity of Ovarian Cancer Cells to Cisplatin
    Article Snippet: The primary antibodies for western blotting are as follows: Anti-Bcl-2 (sc-130307), anti-Bcl-x L (sc-8392), anti-Mcl-1 (sc-69839), anti-NOXA (sc-30209), anti-p53 (DO-1), Anti-p73 (H-79), anti-p21 Waf1/Cip1 and anti–β-actin (Santa Cruz, CA); anti-Bax N-20 (sc-493); anti–caspase-3, anti–cleaved caspase-3, anti–caspase-9 and its cleaved form (Cell Signaling Technology, Danvers, MA); anti-Smac (clone FKE02, R&D Systems, Minneapolis, MN); anti-Cyt C (sc-13156), cytochrome oxidase subunit IV (Molecular Probes). .. Both pcDNA3.1(pc3.1) and pcDNA3.1 plasmid encoding Human NOXA gene (pcDNA3.1-hNOXA, pc3.1-hNoxa) were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously . pc3.1-Bax constructs were generated according to our previous methods . .. Small interfering RNA (siRNA) oligonucleotides were purchased from Dharmacon (Lafayette, CO) with sequences targeting Bax (5′-AACUGAUCAGA ACCAUCAUGG-3′) and Smac (5′-AACCCUGUGUGCGGUUCCUAU-3′).

    Construct:

    Article Title: NOXA-Induced Alterations in the Bax/Smac Axis Enhance Sensitivity of Ovarian Cancer Cells to Cisplatin
    Article Snippet: The primary antibodies for western blotting are as follows: Anti-Bcl-2 (sc-130307), anti-Bcl-x L (sc-8392), anti-Mcl-1 (sc-69839), anti-NOXA (sc-30209), anti-p53 (DO-1), Anti-p73 (H-79), anti-p21 Waf1/Cip1 and anti–β-actin (Santa Cruz, CA); anti-Bax N-20 (sc-493); anti–caspase-3, anti–cleaved caspase-3, anti–caspase-9 and its cleaved form (Cell Signaling Technology, Danvers, MA); anti-Smac (clone FKE02, R&D Systems, Minneapolis, MN); anti-Cyt C (sc-13156), cytochrome oxidase subunit IV (Molecular Probes). .. Both pcDNA3.1(pc3.1) and pcDNA3.1 plasmid encoding Human NOXA gene (pcDNA3.1-hNOXA, pc3.1-hNoxa) were purified by two rounds of passage over EndoFree columns (Qiagen, Chatsworth, CA), as reported previously . pc3.1-Bax constructs were generated according to our previous methods . .. Small interfering RNA (siRNA) oligonucleotides were purchased from Dharmacon (Lafayette, CO) with sequences targeting Bax (5′-AACUGAUCAGA ACCAUCAUGG-3′) and Smac (5′-AACCCUGUGUGCGGUUCCUAU-3′).



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